Total Protein Western Blots
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Introduction
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The Total Protein Western Blots are specifically designed for proteomics, gene discovery, and functional analysis. All products are manufactured using high quality proteins from a wide variety of human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, and plant tissues.
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Features
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High sensitivity and low background
Normalized based on amount of protein used in blots
Interblot control built into every blot
Broad selections of a wide variety of tissues
Suitable for both radioactive and non-radioactive detection
Documentation of tissues' clinical histories available upon request
Identical format Northern blots and tissue paraffin section panels are available. cDNAs from the same tissues are also available for PCR detection.
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Applications |
Identifying specific protein expression in a wide variety of tissues
Determining protein size
Analyzing protein expression pattern
Comparing expression levels of novel proteins
Examining alternative splicing and premature termination of a specific protein
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Description |
Total protein western blots are manufactured with high quality proteins from documented tissues. The tissues are excised, immediately frozen, and then pathologically identified. Proteins are isolated from these tissues using CytoMol's proprietary techniques. The proteins are separated by 4-20% gradient SDS-PAGE gel electrophoresis, and transferred onto a PVDF membrane. Human placenta protein is used as an interblot control lane in each blot for protein expression comparison between different blots. A 10 kd to 250 kd prestained protein marker can be visualized on each blot. These blots are suitable for use in both radioactive and non-radioactive detection. Total protein western blots are supplied ready for use, so that you can immediately screen the protein of interest without the hassle of obtaining hard to find tissues and processing the proteins yourself.
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Storage Conditions |
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Store blots at 4ºC in sealed bag, away from light
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