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Recombinant protein production is a core activity for groups involved in high throughput screening, functional validation and structural biology. It is however a complex and unpredictable process, which can put severe pressure on central facilities or individual researchers, particularly as they may be faced with producing large numbers of diverse proteins.

  CytoMol Unimed Recombinant Protein Expression Service is a complete solution for your needs, helping your organization to deal with the protein bottleneck. The service combines a variety of expression systems (bacterial, baculorvirus, yeast and mammalian cells), across a range of scales, with a full range of purification options, providing you with the material that you require for your application. The service spans vector construction through to purification of ready to use protein and can be tailored to meet your exact requirements.


Construct Generation:
 Clone coding sequence into one of a wide range of expression vectors
 The expression construct will be fully sequence verified
 Preparation of plasmid construct DNA


Assessment and Optimization:
 Introduce plasmid construct DNA into bacterial/mammalian cells
 Selection of over-expressing cells
 Small scale expression and purification study assessed by SDS-PAGE and 

       Western blot (anti-tag or specific antiserum) analysis
 Estimation of protein yield


Scale-up and Purification:
 
Large-scale growth of over-expressing cells
 Cells are harvested and lysed
 Protein typically purified from cleared lysate via       
  purification tag
 Further purification to remove any contaminants e.g. gel   
  filtration
 SDS-PAGE and Western blot analysis of purified protein
 Removal of purification tag and further SDS-PAGE analysis
 Determination of protein concentration
 Delivery of purified protein ready for use in your application


Recombinant Protein Production Team
 Your project will be managed by a team with extensive experience in the expression and purification of a wide range of proteins in both bacterial and mammalian systems. All of our services are fully supported by scientists who directly manage your project and are on hand to develop and support a service that meets your exact requirements.


Cat. # Service Name Size List Price Product Detail
spr45010 Protein expression in E. coli expression vectors, gene construction, modification and production each $1200 Details...
spr45020 Protein expression in baculovirus expression vectors, gene construction, modification and production each $2500 Details...
spr45030 Protein expression in yeast expression vectors, gene construction, modification and production each $2500 Details...
spr45040 Protein expression in mammalian expression vectors, gene construction, modification and production each $2500 Details...
spr45110 Protein expression in E. coli expression vectors, Screening and identify high-efficiency expression individuals each $1800 Details...
spr45120 Protein expression in baculovirus expression vectors, Screening and identify high-efficiency expression individuals each $4500 Details...
spr45130 Protein expression in yeast expression vectors, Screening and identify high-efficiency expression individuals each $4500 Details...
spr45140 Protein expression in mammalian expression vectors, Screening and identify high-efficiency expression individuals each $8600 Details...
spr45210 Protein expression in E. coli expression vectors, Expression optimizations each $4500 Details...
spr45220 Protein expression in baculovirus expression vectors, Expression optimizations each $8600 Details...
spr45230 Protein expression in yeast expression vectors, Expression optimizations each $8600 Details...
spr45240 Protein expression in mammalian expression vectors, Expression optimizations each $28000 Details...
spr45310 Protein expression in E. coli expression vectors, Purify proteins from 1 liter culture with Tag each $1200 Details...
spr45320 Protein expression in baculovirus expression vectors, Purify proteins from 1 liter culture with Tag each $2500 Details...
spr45330 Protein expression in yeast expression vectors, Purify proteins from 1 liter culture with Tag each $2500 Details...
spr45340 Protein expression in mammalian expression vectors, Purify proteins from 1 liter culture with Tag each $3600 Details...
spr45410 Protein expression in E. coli expression vectors, Identify protein by customer's antibody each $800 Details...
spr45420 Protein expression in baculovirus expression vectors, Identify protein by customer's antibody each $800 Details...
spr45430 Protein expression in yeast expression vectors, Identify protein by customer's antibody each $800 Details...
spr45440 Protein expression in mammalian expression vectors, Identify protein by customer's antibody each $800 Details...
spr45460 1D Electrophoresis Services each $800 Details...
spr45470 Enzyme Kinetics Assessment Services, inquiry for details. each $3400 Details...
spr45480 Western Blotting Services each $500 Details...
spr45510 Protein expression in E. coli expression vectors, Scale up, protein expression in 10 liter, pellet only each $1800 Details...
spr45520 Protein expression in baculovirus expression vectors, Scale up, protein expression in 10 liter, pellet only each $3400 Details...
spr45530 Protein expression in yeast expression vectors, Scale up, protein expression in 10 liter, pellet only each $2400 Details...
spr45540 Protein expression in mammalian expression vectors, Scale up, protein expression in 10 liter, pellet only each $6400 Details...
spr45610 Protein expression in E. coli expression vectors, Scale up, protein expression in 10 liter, with tag purification each $5400 Details...
spr45620 Protein expression in baculovirus expression vectors, Scale up, protein expression in 10 liter, with tag purification each $9500 Details...
spr45630 Protein expression in yeast expression vectors, Scale up, protein expression in 10 liter, with tag purification each $7400 Details...
spr45640 Protein expression in mammalian expression vectors, Scale up, protein expression in 10 liter, with tag purification each $14600 Details...
spr45710 Protein expression in E. coli expression vectors, Scale up, protein expression in 40 liter, with tag purification each $16200 Details...
spr45720 Protein expression in baculovirus expression vectors, Scale up, protein expression in 40 liter, with tag purification each $32500 Details...
spr45730 Protein expression in yeast expression vectors, Scale up, protein expression in 40 liter, with tag purification each $22200 Details...
spr45740 Protein expression in mammalian expression vectors, Scale up, protein expression in 40 liter, with tag purification each $38500 Details...
spr45810 Protein expression in E. coli expression vectors, per mg after evaluation 1 mg $3000 Details...
spr45820 Protein expression in baculovirus expression vectors, per mg after evaluation 1 mg $8000 Details...
spr45830 Protein expression in yeast expression vectors, per mg after evaluation 1 mg $6000 Details...
spr45840 Protein expression in mammalian expression vectors, per mg after evaluation 1 mg $15000 Details...
spr45910 Protein expression in E. coli expression vectors, antibody production included each $3600 Details...
spr45920 Protein expression in baculovirus expression vectors, antibody production included each $7800 Details...
spr45930 Protein expression in yeast expression vectors, antibody production included each $4800 Details...
spr45940 Protein expression in mammalian expression vectors, antibody production included each $12800 Details...
spr49200 Peptide Mapping Services each $450 Details...
spr49300 Protein Conjugation Services each $280 Details...
spr49400 Custom Gene Expression Arrays Services, 96 membrane format. Inquiry for other type each $6200 Details...