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Mega Western Protein Arrays

 

Introduction
    Mega Western Protein Array is a novel tool for analysis of protein expressions, combining the unique features of tissue protein micro-arrays and Western blots. Using Mega Western Protein Array, not only protein expressions but also protein sizes can be determined in more than 30 different tissues. The dimensions of CytoMol’s Mega Western Protein Array are only 1x2 inches, and the detection method is the same as is commonly employed for Western blot analysis.
Features
  • Reproducible results - The product comes in an array format. Any two membranes are nearly identical.
  • Higher sensitivity - Protein species are highly concentrated per unit area on the membrane and are readily accessible to antibodies.
  • Versatile - Suitable for both radioactive and non-radioactive detections.
  • Easy-to-use - The membrane can be treated/handled the same as a conventional Western blot.
  • Economical - One Mega Western Protein Array analysis reveals protein expressions in 30 different tissues.
  • Antibody saving - Save at least 4 times of the antibody in compared with using traditional western blot.
  • Stable - Dried storage condition at room temperature
Applications
  • Identification of tissue-specific protein expressions in a wide variety of tissues.
  • Pattern analysis of protein expressions.
  • Comparison of expression levels of target proteins.
  • Determination of the size and relative abundance of target proteins in different tissues.
  • Examination of protein iso-forms due to alternative splicing and premature termination of specific gene transcripts, and post-translational modifications.
Description
    CytoMol’s Mega Western Protein Array was developed using a patent-pending proprietary technology. The Mega Western Protein Arrays are manufactured using high quality denatured protein lysates from documented tissue sources. A protein sample from each tissues is well electrophoresesed along with a Protein molecular weight marker on a large size SDS-PAGE gel and eluted into 24 fractions. Each protein fraction covers a defined molecular weight range. The recovered protein fractions were printed on modified nitrocellulose membranes. Each Mega Western Protein Array is capable of containing as many as hundreds of lanes, and each lane is composed of 24 fractions. These Mega Western Protein Arrays are suitable for use with both radioactive and non-radioactive methods. A user's manual is included. The Mega Western Protein Array is supplied ready to use, so that you can immediately screen proteins of interest without the hassle of obtaining hard to find tissues and processing the proteins yourself.
Storage Conditions
Store the product at 4°C
Cat. # Product Name Size List Price Product Detail
PWB8234480(Manual) Mega Western Protein Array: Adult Huamn tissues from Adipose, Bladder, Brain, Breast, Cerebellum, Cervix, Colon, Diaphragm, Duodenum, Esophagus, Gall Bladder, Heart, Ileum, Jejunum, Kidney, Liver, Lung, Ovary, Pancreas, Placenta, Rectum, Skeletal Muscle, Skin, Spleen, Stomach, Testis, Thymus, Thyroid, Tonsil, Uterus. 1 $799 Details...
PWB8134035(Manual) Mega Western Protein Array: Adult Brains from Human Rabbit, Guinea Pig, Goat, Sheep, Hamster, Chicken, Bovine, Horse, Dog, Porcine. 1 $499 Details...
PWB8134149(Manual) Mega Western Protein Array: Liver and other tissues from  Multiple Species. Ask for availability 1 $499 Details...